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KMID : 0545120230330060797
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Journal of Microbiology and Biotechnology
2023 Volume.33 No. 6 p.797 ~ p.805
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Alpha-Glucosidase Inhibitory Activity of Saponins Isolated from Vernonia gratiosa Hance
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Pham Van Cong
Hoang Le Tuan Anh
Le Ba Vinh
Han Yoo-Kyong
Nguyen Quang Trung
Bui Quang Minh
Ngo Viet Duc
Tran Minh Ngoc
Nguyen Thi Thu Hien
Hoang Duc Manh
Le Thi Lien
Lee Ki-Yong
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Abstract
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Species belonging to the Vernonia (Asteraceae), the largest genus in the tribe Vernonieae (consisting of about 1,000 species), are widely used in food and medicine. These plants are rich sources of bioactive sesquiterpene lactones and steroid saponins, likely including many as yet undiscovered chemical components. A phytochemical investigation resulted in the separation of three new stigmastane-type steroidal saponins (1 ? 3), designated as vernogratiosides A?C, from whole plants of V. gratiosa. Their structures were elucidated based on infrared spectroscopy (IR), one-dimensional (1D) and two-dimensional nuclear magnetic resonance (2D NMR), high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and electronic circular dichroism analyses (ECD), as well as chemical reactivity. Molecular docking analysis of representative saponins with α-glucosidase inhibitory activity was performed. Additionally, the intended substances were tested for their ability to inhibit α-glucosidase activity in a laboratory setting. The results suggested that stigmastane-type steroidal saponins from V. gratiosa are promising candidate antidiabetic agents.
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KEYWORD
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Vernonia gratiosa, stigmastane-type steroidal glycoside, α-glucosidase inhibitor, vernogratiosides A?C
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